Regulatory
Part:BBa_K1104208:Design
Designed by: Ting-Yun Chiang Group: iGEM13_NYMU-Taipei (2013-09-27)
DsbGp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because AhpC promoter(K362001) on PartRegistry is a hybrid promoter containing AhpC1, AhpC2, and DsbG promoter in its 1000bp, we design the new part: AhpC1 and test it apart.
Primers For PCR
| DsbGp_FP | gcttctagag cgattcggctaatgcgtttccat |
| DsbGp_RP | gga ctgcagcggccgctactagta tggaaagggggccattttactttttatc |
PCR Time Protocol
| Temperature(°C) | Time(sec) | Number of Cycles |
|---|---|---|
| 94 | 120 | -- |
| 94 | 15 | 35 |
| 53 | 30 | |
| 72 | 20 | |
| 72 | 300 | -- |
Source
E. coli MG1655 genomic sequence